During the Plio-Pleistocene the Barbary macaque, Macaca sylvanus Linnaeus, 1758 was widely distributed throughout Europe and North Africa (Szalay and Delson, 1979; Delson, 1980; Rook et al., 2001), and it became extinct in Europe during the Late Pleistocene (Elton and O’Regan, 2014). Nowadays this primate is still present in North Africa and a small population has been reintroduced at Gibraltar (Modolo et al., 2005). The taxonomy of the Plio-Pleistocene European macaques is still debated but many authors agree in considering all the fossils as belonging to the M. sylvanus lineage, while the Pleistocene endemic M. majori Azzaroli, 1946 from Sardinia (Italy) has been considered a distinct valid species (Rook and O’Higgins, 2005). The occurrence of this species is documented from Late Pliocene to Late Pleistocene sites located in Northern and Central Italy. Here we describe unpublished dentognathic remains of Macaca sylvanus from the Middle Pleistocene site of Quecchia Quarry (Botticino, Brescia, North Italy).