Roads are fast becoming one of the leading causes of mortality in a number of mammalian species. Between April 2008 and November 2010, 227 km of road between Cork and Caherlistrane, Co. Galway in Southern Ireland were monitored for mammalian road kill. A further 32.5 km, between Cork city and Bandon Co. Cork, was screened from January 2009 to November 2010. In total 45815 km were surveyed over the three year period. In this time 548 mammal fatalities were observed, representing 1.20 per 100 km. Rabbits, hedgehogs, badgers and foxes were the four most common fatalities on both stretches of road, constituting 78% of the mammals killed. May, August and September were the months in which the greatest numbers of casualties were observed. Peaks in fatalities varied on a species basis and coincided with breeding and dispersal patterns.