Suncus murinus, a widespread Soricidae, has been introduced to several islands, strongly impacting endemic species. Its dietary habits, especially at its introduction sites, are poorly understood and the level of impact on native taxa remains unknown. Obtaining dietary samples from shrews is challenging because of their high metabolic rates and rapid digestion. We tested the effectiveness of stomach swabs in analysing the diet of S. murinus using DNA metabarcoding on 300 individuals from Reunion Island (Western Indian Ocean). Non-target DNA amplification was substantial. We identified five preys belonging to three classes of Arthropoda (Arachnida, Insecta and Malacostraca) and one class of Annelida (Clitellata), with two of them assigned to species level, Amynthas rodericensis and Pycnoscelus surinamensis. Lycosidae and Malacostraca were the most frequent groups, each with a 50% frequency of occurrence. Stomach swabs provide insights into the dietary composition of S. murinus, but low DNA yield and purity limited detailed resolution. We highlight the importance of reducing the time lag between trapping and sample extraction and the use of blocking primers to prevent non-target amplification to enhance resolution of S. murinus’ diet composition.