Rapid eradication is an effective management strategy when introduced populations are still localized. This report details the eradication of a small Eastern grey squirrel (Sciurus carolinensis) nucleus in Turin Province, northwestern Italy. The eradication began fifteen months after the first animal was discovered, allowing time to develop and approve the management plan. Following a trial period in August with no captures, from October 2012 to February 2013 16 grey squirrels were removed during monthly sessions from a 258-hectare area. A total of 8-10 cage traps were deployed over 32 days, with a monthly trapping effort of 40 to 70 trap-days and a total of 312 trap-days. Personnel costs amounted to €753, while transport expenses totalled €611. The overall eradication costs reached €2,077. The swift eradication of this grey squirrel nucleus prevented the species from colonizing the largest remaining lowland forest in northern Italy, which is currently home to the native red squirrel. The success of this action was due to the rapid response, which was supported by an existing LIFE project aimed at developing new approaches for grey squirrel management. This project provided trained personnel, materials, and protocols. To achieve similar rapid eradication success, it is crucial to have a well-organized system in place, with trained staff, resources, and intervention procedures ready to be deployed. Streamlining administrative procedures by allowing direct reference to national action plans can expedite rapid response efforts by reducing bureaucratic delays.