Large carnivore mammals are one of the indicators for healthy ecosystems. Therefore, it is important to gather scientific data about carnivores for comprehensive conservation plans. Red fox is one of the carnivore species with widest distribution in Anatolia. In the present study habitat use of red fox was investigated to contribute to reduce the insufficiency of knowledge about carnivores in Turkey. Scent-station method was used to reveal relative habitat use of red fox in Köprülü Canyon National Park in southern Anatolia, which is one of the largest protected areas in the Mediterranean region of Turkey and covers the important habitat types of Mediterranean ecosystem. Anatolian black pine habitat found to be the most used habitat type by red fox among four different habitat types studied in the National Park. However no significant relation could be detected between red fox visits and vegetation structure and elevation. Therefore, the results revealed that red fox behaves as a habitat generalist in Köprülü Canyon National Park.