Abstract As part of the Abruzzo National Park wildlife health management program, serum samples from 62 free-living and captive Abruzzo chamois, Rupicapra (pyrenaica) ornata, were tested to estimate antibody presence to some pathogenous agents. The serum, drawn during chemical immobilisations for introductions or population study programs, were tested for: bovine herpes virus 1 (BHV-1), parainfluenza virus (PI3), pesti virus, foot and mouth disease virus (FMD) type O-A-C, bovine leukemia virus (BLV), ovicaprini lentivirus, encephalomyocarditis virus (EMCV), S-phase brucellae, leptospira interrogans, mycobacterium paratuberculosis, chlamydia psittaci, coxiella burnetii, rickettsia mooseri and conori and toxoplasrna gondii. Twenty-three subjects were found positive to BHV-1 (7/62), pestivirus (6/62), EMCV (8/62), leptospira (5/62) and toxoplasma (4/62). Results did not indicate any active infection but only contact with some pathogenic agents.